Tryptic Soy Broth (Soybean-Casein Digest Broth)

Tryptic Soy Broth (Soybean-Casein Digest Broth)

$160.00

100 g, 500g, 2 kg
Number of sizes: 3
Type: Powder

Dehydrated Powder

Sub-products

Product Name Description SKU Price Action
Tryptic Soy Broth (Soybean-Casein Digest Broth) 9 mL, 90 mL, Custom Number of sizes: 3 Type: Liquid

Prepared Media - Tubes

 TSB-M $35.00 View Options
Tryptic Soy Broth (Soybean-Casein Digest Broth) 100 g, 500g, 2 kg Number of sizes: 3 Type: Powder

Dehydrated Powder

 TSB-G $15.00 View Options
SKU: TSB Categories: ,

Description

Intended Use and Principle of Procedure
Tryptic Soy Broth (Soybean-Casein Digest Broth) is used for the isolation and cultivation of non fastidious and fastidious microorganisms. It is not the medium of choice for anaerobes. The 150 × 15 mm-style plates of Trypticase Soy Agar are convenient for use with Taxo factor strips in the isolation and differentiation of Haemophilus species. Sterile Pack and Isolator Pack plates are useful for monitoring surfaces and air in clean rooms, Isolator Systems, and other environmentally-controlled areas when sterility of the medium is of importance.

Hycheck hygiene contact slides are used for assessing the microbiological contamination of surfaces and fluids.

The combination of casein and soy peptones in TSA renders the medium highly nutritious by supplying organic nitrogen, particularly amino acids and longer-chained peptides. The sodium chloride maintains osmotic equilibrium. Agar is the solidifying agent.

Haemophilus species may be differentiated by their requirements for X and V factors. Paper strips impregnated with these factors are placed on the surface of the medium after inoculation with the test organism. Following incubation, a zone of growth around the strip indicates a requirement for the factor(s).

 

Summary and Explanation
The nutritional composition of TSA has made it a popular medium for many years. It is the medium specified as Soybean-Casein Digest Agar Medium in the USP for the total aerobic microbial count portion of the microbial limit testing procedures. The medium is used for a multitude of purposes, including maintenance of stock cultures, plate counting, isolation of microorganisms from a variety of specimen types, and as a base for media containing blood. It is included in the Bacteriological Analytical Manual of Methods for the Examination of Water, Wastewater, and Foods and is used for testing bacterial contaminants in cosmetics.

 

Formulae
 

Pancreatic Digest of Casein 15.0g
Enzymatic Digest of Soybean Meal 5.0g
Sodium Chloride 5.0g
Agar 15.0g

 

Procedure
Swab specimens may be inserted into the medium after inoculation of appropriate plated media. For liquid specimens, use a sterile inoculating loop to transfer a loopful of the specimen to the broth medium. Specimens known or suspected to contain obligate anaerobes should be inoculated near the bottom of the tube.

Incubate the tubes and bottles with loosened caps at 35 ± 2°C aerobically with or without supplementation with carbon dioxide. Tubed and bottled media intended for the cultivation of anaerobes should be incubated under anaerobic conditions. An efficient and easy way to obtain suitable anaerobic conditions is through the use of BBL GasPak or GasPak EZ anaerobic systems or equivalent alternative systems.

Examine for growth after 18-24 hours and 42-48 hours of incubation.

For use in sterility testing, consult the USP for procedural details and specifications for the volume of the medium relative to container size.

For use in the preparation of standardized inocula for antimicrobial susceptibility testing, refer to the NCCLS standards.

 

Directions for Preparation from Dehydrated Product
1.    Suspend the powder in 1 L of purified water:

             Bacto Tryptic Soy Broth – 30 g;

             BBL Trypticase Soy Broth – 30 g;

             Bacto Tryptic Soy Broth without Dextrose – 27.5 g. Mix thoroughly.

2.    Warm gently until the solution is complete.

3.    Autoclave at 121°C for 15 minutes.

4.    Test samples of the finished product for performance using stable, typical control cultures.

 

Identification Specification and Cultural Response
 

Dehydrated Appearance: Light beige, free-flowing, homogeneous.
 

 

Prepared Appearance:

 

 

Solution:

  

 

Light amber, clear.

 

3.0% solution, soluble in purified water upon warming. Solution is light amber, clear.
 

pH:

 

 7.3 ± 0.2
Cultural Response: 

 

 Prepare the medium per label directions.  Inoculate and incubate at 30-35°C for 18-48 hours (up to 72 hours, if necessary). To test for growth promotion according to the USP/EP, inoculate using organisms marked with (*) and incubate at 20-25°C for 3 days and 7 days for bacteria and fungi, respectively (incubate B. subtilis at 20-25°C and 30-35°C).
Reaction 3.0%
Solution 25°C

 

 

Microorganism ATCC CFU Recovery Plain/SB USP/EP Growth
Neisseria meningitidis 13090 102-102 Fair to Good N/A
Staphylococcus epidermidis 12228 102-102 Good N/A
Streptococcus pneumoniae 6305 102-102 Good N/A
Streptococcus pyogenes 19615 102-102 Good N/A
Aspergillus niger 16404 102-102 N/A Growth
Bacillus subtilis* (20-25°C) 6633 102-102 N/A Growth
Bacillus subtilis* (30-35°C) 6633 102-102 N/A Growth
Candida albicans* 10231 102-102 N/A Growth

 

Expected Results
Growth in broth media is indicated by the presence of turbidity compared to an uninoculated control. Broth cultures should be held for at least a week before discarding as negative.

 

References
  1. MacFaddin. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria, vol. 1. Williams & Wilkins, Baltimore, Md.
  2. Marshall (ed.). 1993. Standard methods for the examination of dairy products, 16th ed. American Public Health Association, Washington, D.C.
  3. Forbes, Sahm and Weissfeld. 1998. Bailey & Scott’s diagnostic microbiology, 10th ed. Mosby, Inc. St. Louis, Mo.
  4. Fredette and Forget. 1961. The sensitivity of several media to small inocula. Extract from a paper presented at the Canadian Society of Microbiology Annual Meeting, June 12-15. Kingston, Ontario, Canada.
  5. United States Pharmacopeial Convention, Inc. 2001. The United States pharmacopeia 25/The national formulary 20 – 2002. United States Pharmacopeial Convention, Inc., Rockville, Md.
  6. Federal Register. 1992. Fed. Regist. 21:113.26.
  7. Clesceri, Greenberg, and Eaton (ed.). 1998. Standard methods for the examination of water and wastewater, 20th ed. American Public Health Association, Washington, D.C.
  8. Curry, Joyce, and McEwen. 1993. CTFA microbiology guidelines. The Cosmetic, Toiletry and Fragrance Association, Inc., Washington, D.C.
  9. U.S. Food and Drug Administration. 1995. Bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, Md.
  10. Horwitz (ed.). 2000. Official methods of analysis AOAC International, 17th ed., vol. 1. AOAC International, Gaithersburg, Md.
  11. Downes and Ito (ed.). 2001. Compendium of methods for the microbiological examination of foods, 4th ed. American Public Health Association, Washington, D.C.
  12. National Committee for Clinical Laboratory Standards. 2000. Approved standard: M2-A7. Per- formance standards for antimicrobial disk susceptibility tests, 7th ed., NCCLS, Wayne, Pa.
  13. National Committee for Clinical Laboratory Standards. 2000. Approved standard: M7-A5. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, 5th ed. NCCLS, Wayne, Pa.
  14. Facklam, Sahm and Teixeira. 1999. In Murray, Baron, Pfaller, Tenover and Yolken (ed.), Manual of clinical microbiology, 7th ed. American Society for Microbiology, Washington, D.C.

15.  Fildes. 1920. Br. J. Exp. Pathol. 1:129.

 

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