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Tryptic Soy Broth (Soybean-Casein Digest Broth)

Name: Tryptic Soy Broth (Soybean-Casein Digest Broth)
SKU: TSB-M
Availability: InStock

Tryptic Soy Broth (Soybean-Casein Digest Broth)

$35.00 – $160.00

9 mL, 90 mL, Custom
Number of sizes: 3
Type: Liquid

Prepared Media – Tubes

No sub-products available for this product.

SKU: TSB-M Category: Ready To Use – Tubes

Description

Intended Use and Principle of Procedure

Tryptic (Trypticase) Soy Broth (Soybean-Casein Digest Broth) conforms with the specifications of The United States Pharmacopeia (USP).

Tryptic (Trypticase) Soy Broth (Soybean-Casein Digest Medium) is a general-purpose medium used in qualitative procedures for the cultivation of fastidious and non fastidious microorganisms from a variety of clinical and nonclinical specimens.

Trypticase Soy Broth with 6.5% Sodium Chloride is used to differentiate Enterococcus spp. from the Streptococcus bovis group of streptococci.

Trypticase Soy Broth with 5% Fildes Enrichment is used for the cultivation of fastidious organisms, e.g., Haemophilus influenzae.

Enzymatic digests of casein and soybean meal provide amino acids and other complex nitrogenous substances. Dextrose is an energy source. Sodium chloride maintains the osmotic equilibrium. Dibasic potassium phosphate acts as a buffer to control pH.

The addition of 6.5% sodium chloride to Trypticase Soy Broth permits the differentiation of salt-tolerant enterococci, which are resistant to the high salt content, from the salt-intolerant S. bovis group and other streptococcal species. At this concentration, sodium chloride is a selective agent that interferes with membrane permeability and osmotic and electrokinetic equilibria.

Fildes Enrichment is a peptic digest of sheep blood that sup- plies the X (hemin) and V (nicotinamide adenine dinucleotide, NAD) factors necessary for the growth of H. influenzae.

Dextrose is omitted from the formula for Tryptic Soy Broth Dextrose to permit the use of the medium in fermentation studies. The carbohydrate concentration used most frequently in fermentation reactions is 0.5% or 1%.

Tryptic Soy Broth and Trypticase Soy Broth are provided as prepared media in a variety of bottle styles. In addition, Tryptic Soy Broth is provided as a Sterile Pack Bottle; i.e., the bottle has been terminally sterilized inside autoclavable double bags. All varieties of bottled TSB conform with requirements for Ready-To-Use Media as described in the USP.

Summary and Explanation

Tryptic (Trypticase) Soy Broth (TSB) is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative, and anaerobic bacteria and fungi.1-4 Because of its capacity for growth promotion, this formulation is included in the USP as a sterility test medium and for use in performing microbial limit testing.

TSB was chosen by the USDA Animal and Plant Health Inspection Service to detect viable bacteria in live vaccines.6 It is used in the coliphage detection procedure, a methodology in Standard Methods for the Examination of Water and Wastewater. TSB is recommended for testing bacterial contaminants in cosmetics8 and complies with established standards in the food industry.

TSB is also recommended, because of growth promotion, for use as the inoculum broth for disc diffusion and agar dilution antimicrobial susceptibility testing as standardized by the National Committee for Clinical Laboratory Standards (NCCLS).

Trypticase Soy Broth with 6.5% Sodium Chloride is used to differentiate the enterococcal species from the S. bovis group of streptococci by the 6.5% NaCl tolerance test.

Trypticase Soy Broth supplemented with 5% Fildes Enrich- ment provides growth factors necessary for the cultivation of fastidious organisms.

Tryptic Soy Broth without Dextrose, a modification of TSB, is a basal medium to which carbohydrates may be added for use in fermentation studies. Phenol red and other indicators may also be added.

Formulae

Pancreatic Digest of Casein	17.0g
Enzymatic Digest of Soybean Meal	3.0g
Sodium Chloride	5.0g
Dipotassium Phosphate	2.5g
Dextrose	2.5g

Procedure

Swab specimens may be inserted into the medium after inoculation of appropriate plated media. For liquid specimens, use a sterile inoculating loop to transfer a loopful of the specimen to the broth medium. Specimens known or suspected to contain obligate anaerobes should be inoculated near the bottom of the tube.

Incubate the tubes and bottles with loosened caps at 35 ± 2°C aerobically with or without supplementation with carbon dioxide. Tubed and bottled media intended for the cultivation of anaerobes should be incubated under anaerobic conditions. An efficient and easy way to obtain suitable anaerobic conditions is through the use of BBL GasPak or GasPak EZ anaerobic systems or equivalent alternative systems.

Examine for growth after 18-24 hours and 42-48 hours of incubation.

For use in sterility testing, consult the USP for procedural details and specifications for the volume of the medium relative to container size.

For use in the preparation of standardized inocula for antimicrobial susceptibility testing, refer to the NCCLS standards.

Directions for Preparation from Dehydrated Product

1. Suspend the powder in 1 L of purified water:

Bacto Tryptic Soy Broth – 30 g;

BBL Trypticase Soy Broth – 30 g;

Bacto Tryptic Soy Broth without Dextrose – 27.5 g. Mix thoroughly.

2. Warm gently until the solution is complete.

3. Autoclave at 121°C for 15 minutes.

4. Test samples of the finished product for performance using stable, typical control cultures.

Identification Specification and Cultural Response

Dehydrated Appearance:	Light beige, free-flowing, homogeneous.
Prepared Appearance: Solution:	Light amber, clear. 3.0% solution, soluble in purified water upon warming. Solution is light amber, clear.
pH:	7.3 ± 0.2
Cultural Response:	Prepare the medium per label directions. Inoculate and incubate at 30-35°C for 18-48 hours (up to 72 hours, if necessary). To test for growth promotion according to the USP/EP, inoculate using organisms marked with (*) and incubate at 20-25°C for 3 days and 7 days for bacteria and fungi, respectively (incubate B. subtilis at 20-25°C and 30-35°C).
Reaction	3.0%
Solution	25°C

Microorganism	ATCC	CFU	Recovery Plain/SB	USP/EP Growth
Neisseria meningitidis	13090	102-102	Fair to Good	N/A
Staphylococcus epidermidis	12228	102-102	Good	N/A
Streptococcus pneumoniae	6305	102-102	Good	N/A
Streptococcus pyogenes	19615	102-102	Good	N/A
Aspergillus niger	16404	102-102	N/A	Growth
Bacillus subtilis (20-25*°C)	6633	102-102	N/A	Growth
Bacillus subtilis (30-35*°C)	6633	102-102	N/A	Growth
Candida albicans*	10231	102-102	N/A	Growth

Expected Results

Growth in broth media is indicated by the presence of turbidity compared to an uninoculated control. Broth cultures should be held for at least a week before discarding as negative.

References

MacFaddin. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria, vol. 1. Williams & Wilkins, Baltimore, Md.
Marshall (ed.). 1993. Standard methods for the examination of dairy products, 16th ed. American Public Health Association, Washington, D.C.
Forbes, Sahm and Weissfeld. 1998. Bailey & Scott’s diagnostic microbiology, 10th ed. Mosby, Inc. St. Louis, Mo.
Fredette and Forget. 1961. The sensitivity of several media to small inocula. Extract from a paper presented at the Canadian Society of Microbiology Annual Meeting, June 12-15. Kingston, Ontario, Canada.
United States Pharmacopeial Convention, Inc. 2001. The United States pharmacopeia 25/The national formulary 20 – 2002. United States Pharmacopeial Convention, Inc., Rockville, Md.
Federal Register. 1992. Fed. Regist. 21:113.26.
Clesceri, Greenberg, and Eaton (ed.). 1998. Standard methods for the examination of water and wastewater, 20th ed. American Public Health Association, Washington, D.C.
Curry, Joyce, and McEwen. 1993. CTFA microbiology guidelines. The Cosmetic, Toiletry and Fragrance Association, Inc., Washington, D.C.
U.S. Food and Drug Administration. 1995. Bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, Md.
Horwitz (ed.). 2000. Official methods of analysis AOAC International, 17th ed., vol. 1. AOAC International, Gaithersburg, Md.
Downes and Ito (ed.). 2001. Compendium of methods for the microbiological examination of foods, 4th ed. American Public Health Association, Washington, D.C.
National Committee for Clinical Laboratory Standards. 2000. Approved standard: M2-A7. Per- formance standards for antimicrobial disk susceptibility tests, 7th ed., NCCLS, Wayne, Pa.
National Committee for Clinical Laboratory Standards. 2000. Approved standard: M7-A5. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, 5th ed. NCCLS, Wayne, Pa.
Facklam, Sahm and Teixeira. 1999. In Murray, Baron, Pfaller, Tenover and Yolken (ed.), Manual of clinical microbiology, 7th ed. American Society for Microbiology, Washington, D.C.

15. Fildes. 1920. Br. J. Exp. Pathol. 1:129.

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Size	9mL, 90mL, Custom

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Tryptic Soy Broth (Soybean-Casein Digest Broth)

Tryptic Soy Broth (Soybean-Casein Digest Broth)

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